METHODS FOR RAPIDLY TRANSFORMING MONOCOTS

摘要

The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

主权项

1. A method for producing a transgenic corn plant comprising:
a) transforming an explant with at least a first selected DNA via bacterially mediated transformation of the explant; b) culturing the explant in a first culture medium comprising an effective ratio of cytokinin and auxin to promote development of regenerable structures capable of root and/or shoot formation; and c) culturing the explant in at least a second culture medium comprising a reduced amount of auxin relative to the first culture medium, and/or a third culture medium that lacks auxin and cytokinin, wherein the second culture medium and/or third culture medium supports the simultaneous growth of root and shoot tissues, to produce a regenerated transgenic corn plant; wherein the ratio of cytokinin to auxin in the first culture medium is from about 0.005 to about 0.03 (w/w), wherein the first culture medium comprises from about 0.001 mg/L to about 10 mg/L of cytokinin and from about 0.1 mg/L to about 15 mg/L auxin, and wherein the regenerated transgenic corn plant is produced within about 4-8 weeks of transforming the explant.