ULTRA-SENSITIVE CHEMILUMINESCENT SUBSTRATES FOR ENZYMES AND THEIR CONJUGATES

摘要

New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent, an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.

主权项

1. A chemiluminescent assay formulation, comprising:
at least one chemiluminescent compound which is a 1,2-dioxetane coupled acridane corresponding to the formula:wherein R12 a is substituted 1,2-dioxetane, R13, is a linear or branched C1 to C6 alkyl or deuterated alkyl, aryl or deuterated aryl, arylalkyl, alkylaryl, heteroalkyl, alkylalkene, arylalkene, alkylnitrile, alkylalcohol and alkyacid; R14 and R15 may be alkyl or deuterated alkyl, methoxy or deuterated methoxy, Cl, Br or CN, and wherein at least one of R12, R13, R14, and R15 is a deuterium atom or deuterium atom containing organic group and X is oxygen, nitrogen or sulfur.