Automated Intracytoplasmic Sperm Injection Assisted Fertilization System

摘要

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.

主权项

1. An enclosed microfluidics cassette device for intracytoplasmic sperm injection assisted fertilization comprising:
a) an oocyte reservoir comprising an oocyte chamber, an inlet and an outlet; b) an oocyte cumulus removal channel in selective fluid communication with the oocyte reservoir outlet; c) an oocyte immobilizing station in selective fluid communication with the oocyte cumulus removal channel; d) a spermatozoa reservoir comprising a spermatozoa chamber, an inlet and outlet; e) a motile spermatozoa isolating channel in selective fluid communication with the spermatozoa reservoir outlet; f) a motile spermatozoa immobilization station in selective fluid communication with the motile spermatozoa isolating channel and in selective fluid communication with the oocyte immobilization station; and g) an embryo culturing chamber in selective fluid communication with the oocyte immobilization station.