GENE EXPRESSION PROFILING FROM FFPE SAMPLES

摘要

Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.

主权项

1. A method for amplification and analysis of expressed RNA from cells of a deparaffinized FFPE (formalin fixed paraffin embedded) sample, said method comprising:
synthesizing, with a primer comprising a sequence complementary to RNA extracted from said cells of said deparaffinized FFPE sample, to produce first cDNA strands; amplifying said cDNA strands to produce amplified molecules; hybridizing said amplified molecules to a microarray; and producing signals from hybridized molecules, wherein the signals reflect expressed RNA in said cells.