发明名称 |
METHOD, REAGENT AND KIT FOR DETERMINATION OF CHOLESTEROL IN REMNANT-LIKE PARTICLES (RLP) |
摘要 |
<p>The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.</p> |
申请公布号 |
CA2597792(C) |
申请公布日期 |
2015.12.15 |
申请号 |
CA20062597792 |
申请日期 |
2006.02.14 |
申请人 |
KYOWA MEDEX CO., LTD. |
发明人 |
MIYAUCHI, KAZUHITO;FUJINAKA, MAYUMI |
分类号 |
C12Q1/60;C12Q1/26;C12Q1/34;C12Q1/44;G01N21/78;G01N33/92 |
主分类号 |
C12Q1/60 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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