发明名称 |
METHODS OF REDUCING DENSITY-DEPENDENT GC BIAS IN AMPLIFICATION |
摘要 |
The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells. |
申请公布号 |
WO2015185916(A1) |
申请公布日期 |
2015.12.10 |
申请号 |
WO2015GB51605 |
申请日期 |
2015.06.02 |
申请人 |
ILLUMINA CAMBRIDGE LIMITED |
发明人 |
BOUTELL, JONATHAN MARK;SHANAHAN, SUSAN;RIGATTI, ROBERTO |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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