ISOLATED POLYPEPTIDES, KITS COMPRISING THE SAME AND USES THEREOF

摘要

Disclosed herein are isolated polypeptides, kits comprising the same, and uses thereof. The polypeptides are respectively isolated from Arabidopsis thaliana and are capable of catalyzing the cleavage of a peptide linkage located between the C-terminus of the distal ubiquitin (UB) and any of the first methionine (M1), the 48th (K48) or the 63rd lysine (K63) of the proximal UB in a UB chain, which is conjugated to a substrate protein.

主权项

1. An isolated polypeptide capable of cleaving a peptide linkage located between the C-terminus of the distal ubiquitin (UB) and any of the first methionine (M1), the 48th (K48) or the 63rd lysine (K63) of the proximal UB in a UB chain conjugated with a substrate protein, comprising:
an amino acid sequence at least 85% identical to any of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9.