Designer Photoautotrophic and Hydrogenotrophic Production of Alcohols and Biodiesel

摘要

Designer Calvin-cycle-channeled and hydrogenotrophic biofuel-production pathways, the associated designer genes and designer transgenic organisms for autotrophic production of alcohols and biodiesel from carbon dioxide, hydrogen, and/or water are disclosed. The alcohols include methanol, ethanol, propanol, 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer autotrophic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and hydrogenotrophic pathway gene(s) and biosafety-guarding technology for enhanced autotrophic production of alcohols and biodiesel from carbon dioxide and water; wherein the designer transgenic cells in their mass liquid culture can inducibly self-flocculate for enhanced harvesting of their biomass upon the expression of the designer cell surface-linked positively charged polypeptides.

主权项

1. A method for autotrophic production of alcohols and biodiesel comprising:
introducing a transgenic autotrophic organism into a reactor system, the transgenic autotrophic organism comprising transgenes coding for a set of enzymes to confer a photoautotrophic or a hydrogenotrophic pathway for production of alcohol and biodiesel; using a photosynthetic or hydrogenotrophic process in the biological reactor to synthesize the alcohol and biodiesel from carbon dioxide and water; using a product separation process to harvest the synthesized alcohol and biodiesel from the bioreactor; and harvesting biomass from liquid culture in the bioreactor with self-flocculation wherein the designer transgenic cells in their mass liquid culture inducibly self-flocculate for enhanced harvesting of their biomass upon the expression of the designer cell surface-linked positively charged polypeptides.