A NOVEL AFFINITY PEPTIDE LIBRARY OF IGG CONSTRUCTED BASED ON PROTEIN A AFFINITY MODEL AND THE APPLICATION OF THE DESIGN METHOD THEREOF

摘要

An affinity ligand peptide library of IgG constructed on the basis of Protein A affinity model and the application of a design method thereof According to the Molecular Mechanics—Poisson-Boltzmann surface area (MM/PBSA) method and on the basis of the known human IgG-Protein A complex structure, the hot spots of Protein A that have high affinity for human IgG are obtained analytically, and a Protein A simplified affinity model is built thereof. An affinity peptide library of IgG is constructed including heptapeptide and octapeptide structural modes. On the basis of the peptide structural modes, the types of inserted amino acids that ‘X’ residues represent are further identified using amino acid location method. Then, molecular docking and molecular dynamics simulation methods are used to screen the candidate peptides successively. Finally, the affinity peptide ligands that can effectively purify IgG are identified using affinity chromatography.

主权项

1. A novel affinity ligand peptide library of IgG built on the basis of the Protein A affinity model, involving adding a cysteine to the middle region of the peptide, wherein, eight peptide models are included:FYCHXXXE, FYXHCXXE, FYCHXXR, FYXHCXR, FYXCRXE,YFXCRXE, HXYFCXR,andHXYFCXK; ‘X’ represents the common 19 amino acids except cysteine.