EFFICIENT PRODUCTION OF BIOFUELS FROM CELLS CARRYING A METABOLIC-BYPASS GENE CASSETTE

摘要

Increasing the production of a glycolytic intermediate and the production of an organic compound, such as ethanol, by a bacteria expressing a polyglutamine protein is achieved by preparing a feed stream; combining the bacteria and feed stream; fermenting the feed stream; cooling the feed stream during fermentation; recovering the organic compound; and concentrating the organic compound. The feed stream can be a waste stream from an ethanol production facility. The process can allow for a similar yield to the ethanol plant alone but with the use of less feed stock, such as corn, sugarcane, sorghum, cassava, switchgrass, and wood chips.

主权项

1. A process for increasing the production of a glycolytic intermediate and the production of an organic compound by bacteria comprising
preparing a feed stream; combining the bacteria and feed stream; fermenting the feed stream; cooling the feed stream during fermentation; recovering the organic compound; and concentrating the organic compound; wherein the bacteria comprises a nucleic acid molecule comprising a CAG/CAA repeat that encodes a polyglutamine protein that increases production of a glycolytic intermediate; and wherein a starting substrate is selected from at least one of the group consisting of sugar and protein.