Improved protein production in fungi or yeasts

摘要

The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell by overexpressing a polynucleotide encoding a protein which is involved in one or more proteinogenic amino acid biosynthesis pathways, thereby increasing the yield of a protein of interest. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.