METHODS AND COMPOSITIONS FOR DETECTING TARGET NUCLEIC ACIDS
摘要
<p>The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.</p>
申请公布号
EP2710145(B1)
申请公布日期
2015.12.09
申请号
EP20120723799
申请日期
2012.05.17
申请人
DXTERITY DIAGNOSTICS INCORPORATED
发明人
TERBRUEGGEN, ROBERT;LIU, YENBOU;CHILDERS, JOHN, RAY;KIM, CHANG, HEE;ABEDI, MAJID R.