发明名称 |
Method of cloning a lambda light chain variable domain using a human lambda light chain mouse |
摘要 |
Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided. |
申请公布号 |
US9206262(B2) |
申请公布日期 |
2015.12.08 |
申请号 |
US201514638424 |
申请日期 |
2015.03.04 |
申请人 |
Regeneron Pharmaceuticals, Inc. |
发明人 |
Macdonald Lynn;Stevens Sean;Gurer Cagan;Murphy Andrew J.;Meagher Karolina A. |
分类号 |
C12P21/00;A01K67/027;C07K16/46;C07K16/00;C12N15/85;C07K16/18;C12N15/10 |
主分类号 |
C12P21/00 |
代理机构 |
Foley Hoag LLP |
代理人 |
Jones Brendan T.;Miyamoto Neil;Foley Hoag LLP |
主权项 |
1. A method for cloning a λ light chain variable domain, comprising the steps of:
(a) obtaining a cell from a transgenic mouse whose genome comprises an endogenous κ light chain immunoglobulin locus comprising a replacement of endogenous Vκ and Jκ gene segments with human Vλ and Jλ gene segments, wherein the human Vλ and Jλ gene segments are operably linked to a mouse Cκ gene such that the mouse expresses an immunoglobulin light chain that comprises a human λ variable region nucleic acid sequence fused with a mouse κ constant region nucleic acid sequence; (b) cloning a λ light chain variable domain derived from the human Vλ and Jλ gene segments. |
地址 |
Tarrytown NY US |