| 主权项 |
1. A method of detecting the preovulatory Luteinizing Hormone (LH) peak in a biological sample obtained from mammals consisting of the following steps:
(a) contacting a test surface comprising an anti-Luteinizing Hormone (anti-LH) antibody with a buffer solution comprising 5 to 50 vol % of fetal calf serum; (b) contacting said surface obtained in step (a) with a biological sample; (c) after step (b), rinsing the test surface in a washing solution; (d) contacting the test surface rinsed in step (c) with a buffer solution of conjugate comprising an enzyme-coupled anti-LH antibody and from 5 to 50 vol % of fetal calf serum; (e) after step (d), rinsing the test surface in a washing solution; (f) after step (e), contacting the test surface with a solution comprising a substrate of said enzyme, said substrate comprising 3,3′,5,5′-tetramethylbenzidine membrane (Membrane TMB), wherein binding of said substrate and said enzyme causes a color change on the test surface that is proportional to the amount of LH present in the test sample; and (g) visually observing the color change on the test surface to detect the presence of LH in the biological sample. |
