ELISPOT method having two filter systems

摘要

An ELISPOT method for in vitro diagnosis and/or in vitro therapy monitoring of infections and/or infectious diseases comprising incubating eukaryotic cells with an antigen, measuring the number of immunocompetent cells which secrete at least two different cytokines as a reaction to the antigen, and in measuring the immunocompetent cells, visualizing the cells with the aid of at least two different dyes and at least two different filter sets, the filter sets comprising narrowband filters.

主权项

1. An ELISPOT method for in vitro diagnosis of infection by borrelia comprising enriching or separating liquid blood fraction from its cellular components which comprises eukaryotic cells enriched from a blood sample, cervical smear, or bronchial lavage, incubating the eukaryotic cells with a borrelia-specific antigen, contacting immunocompetent cells with at least two capture antibodies and at least two corresponding detection antibodies, wherein each captive antibody and its corresponding detection antibody bind to the same cytokine secreted by the immunocompetent cells as a reaction to the antigen, and wherein the at least two detection antibodies are labeled with a different dye, visualizing the immunocompetent cells by detecting the different dyes and using at least two different filter sets, the filter sets each comprising one narrowband excitation filter and one narrowband blocking filter, wherein the narrowband excitation filter is transmissive for light which is provided for luminescent excitation of a respective dye and the narrowband blocking filter is transmissive for light emitted by a respective dye and measuring the number of immunocompetent cells which secrete at least interferon-γand interleukin-2 as a reaction to the antigen by superimposing at least two two-dimensional images generated during the visualization for avoiding luminescences of different dyes interfering and strong luminescences being superimposed on weak luminescences.