Micro-molded cytometer cartridge with integrated optics

摘要

A cytometer cartridge insert includes a micro-molded component and a plastic laminate component. The micro-molded component is embedded in the plastic laminate component.

主权项

1. An apparatus comprising:
a cytometer cartridge insert, the cytometer cartridge insert comprising:
a micro-molded component; anda plastic laminate component; wherein the micro-molded component is embedded in the plastic laminate component; wherein the micro-molded component comprises:
a sample via comprising a diameter ranging from approximately 20 microns to approximately 100 microns;a measurement channel with an integrated optical lens, the measurement channel coupled to the sample via by way of a lysing and sphering channel, the measurement channel comprising a diameter ranging from approximately 20 microns to approximately 200 microns, the integrated optical lens permitting detection of scatter angles of about 90 degrees; anda cuvette coupled to the sample via by way of a sample channel, the cuvette comprising an optical mask; wherein the integrated optical lens is aligned with the measurement channel such that the measurement channel walls do not produce random scatter noise; and wherein the measurement channel walls have a low RMS planarity level and optical finish characteristics such that an illumination beam on the walls generates light scatter having a same group of angles as the illumination beam, thereby occupying a small solid angle of a budget of angles and not producing the random scatter noise, thereby providing for a cell stream to be close to the measurement channel walls, thereby reducing the need for a sheathing fluid and reducing an amount of liquid waste.