发明名称 |
Stabil opretholdelse af flere kopier af i det mindste to ORF i den samme orientering |
摘要 |
The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome. |
申请公布号 |
DK1766002(T3) |
申请公布日期 |
2015.12.07 |
申请号 |
DK20050750839T |
申请日期 |
2005.06.20 |
申请人 |
NOVOZYMES A/S |
发明人 |
PEDERSEN, POUL, ERIK;JØRGENSEN, STEEN, TROELS |
分类号 |
C12N15/67;C12N1/21;C12N9/52;C12N15/90 |
主分类号 |
C12N15/67 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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