| 发明名称 |
PROCESS OF MODULATING MAN5 AND/OR AFUCOSYLATION CONTENT OF A GLYCOPROTEIN COMPOSITION |
| 摘要 |
Provided is a method for producing glycoprotein composition with an increased percentage of Man5 and/or afucosylated glycans. Use of manganese for increasing the percentage of Man5 and afucosylated glycans in glycoprotein composition is further provided. |
| 申请公布号 |
US2015344579(A1) |
申请公布日期 |
2015.12.03 |
| 申请号 |
US201314374163 |
申请日期 |
2013.01.18 |
| 申请人 |
DR. REDDY'S LABORATORIES LIMITED |
发明人 |
THUDUPPATHY Guruvasuthevan Ramasamy;MADHAVA RAM Paranandi Ananta;BHAVSAR Kaumil;GARG Neha |
| 分类号 |
C07K16/28 |
主分类号 |
C07K16/28 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A cell culture process for obtaining a glycoprotein composition comprising increased percentage of Man5 glycans and/or afucosylated glycans by culturing cells expressing said recombinant glycoprotein in culture medium comprising
a) divalent manganese ion or its salts thereof b) at a pH of about 6.8 to about 7.2 c) recovering the said glycoprotein from the said cell culture. |
| 地址 |
Qutubullapur IN |
