Intracellular Phenotypic Screening

摘要

The present invention relates to a method for identifying a cellular target involved in a cell phenotype comprising identifying an intrabody which can modify a cell phenotype and identifying a direct or indirect cellular target of the intrabody. The present invention also relates to intrabodies 3H2-1, 3H2-VH and 5H4 which are capable of inhibiting the degranulation reaction in mast cells triggered by an allergic stimulus, and especially to intrabodies 3H2-1 and 5H4 which are capable of directly or indirectly targeting a protein of the ABCF1 family and of the C120RF4 family respectively. The present invention also relates to ABCF1 and C120RF4 inhibitors for use in therapy, in particular for treating allergic and/or inflammatory conditions.

主权项

1. Method for identifying a cellular target which is involved in a cell phenotype, comprising:
a) identifying an intrabody comprising a full VH and/or VL domain of an immunoglobulin, which can induce, modify or suppress said phenotype when present inside a cell; b) identifying a cellular target which is a direct or indirect target of said intrabody in said cell; and optionally c) isolating said cellular target.