SYSTEM AND METHOD FOR PICOLITER VOLUME MICROFLUIDIC DIAGNOSTICS

摘要

The present invention provides a method for a picoliter volume microfluidic assay. The method includes the steps of providing a first solution comprising a sensor, preparing a sample comprising at least one analyte, and combining the sample with an indicator, thereby forming a second solution. The method further includes co-encapsulating the first solution and the second solution in a plurality of droplets with a microfluidic device, incubating the plurality of droplets, thereby forming at least one complex comprising the sensor, at least one analyte and indicator, and detecting the at least one complex. A primary signal associated with the at least one complex is distinguishable from a background signal associated with at least one of the sensor, at least one analyte and indicator individually.

主权项

1. A method for a picoliter volume microfluidic assay comprising:
providing a first solution comprising a sensor; preparing a sample comprising at least one analyte; combining the sample with an indicator, thereby forming a second solution; co-encapsulating the first solution and the second solution in a plurality of droplets with a microfluidic device; incubating the plurality of droplets, thereby forming at least one complex comprising the sensor, at least one analyte and indicator; and detecting the at least one complex, wherein a primary signal associated with the at least one complex is distinguishable from a background signal associated with at least one of the sensor, at least one analyte and indicator individually.