TNNT1 MINI-PROMOTERS

摘要

Isolated polynucleotides comprising a TNNT1 mini-promoters are provided. The mini-promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, gene therapy, etc.

主权项

1. An isolated polynucleotide comprising a TNNT1 mini-promoter wherein the TNNT1 mini-promoter comprises one or more TNNT1 regulatory elements with substantial similarity to SEQ ID NO: 7-9 operably joined to a TNNT1 basal promoter with substantial similarity to SEQ ID NO: 5 or 6 through a non-native spacing between the regulatory element and the basal promoter.