Tailored oils

摘要

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.

主权项

1. A triglyceride oil produced by a microalgae, the oil having an altered fatty acid profile relative to an oil produced by a non-recombinant microalgae, comprising 3.5% or less saturated fatty acids, greater than 50% oleic acid, and optionally comprising greater than 1% palmitoleic acid, wherein the microalgae is a recombinant oleaginous cell of the genus Prototheca, comprising an exogenous gene encoding an active ketoacyl-ACP synthase.