Method for treating cellulosic material and CBHII/Cel6A enzymes useful therein

摘要

The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.

主权项

1. A nucleic acid molecule, comprising a polynucleotide sequence selected from the group consisting of:
(a) a polynucleotide sequence encoding a polypeptide having cellobiohydrolase II activity, the polypeptide comprising the sequence consisting of amino acids 1-25 directly linked to amino acids 64-478 of SEQ ID NO:12 or the sequence consisting of amino acids 19-25 directly linked to amino acids 64-478 of SEQ ID NO:12; (b) a polynucleotide sequence encoding a polypeptide having cellobiohydrolase II activity, the polypeptide having at least 80% identity to the sequence consisting of amino acids 1-25 directly linked to amino acids 64-478 of SEQ ID NO:12 or the sequence consisting of amino acids 19-25 directly linked to amino acids 64-478 of SEQ ID NO:12; (c) a polynucleotide sequence comprising a cDNA comprising the coding sequence of the polynucleotide sequence set forth in SEQ ID NO:11; (d) a polynucleotide sequence comprising a cDNA comprising the coding sequence of the polynucleotide sequence encoding a polypeptide having cellobiohydrolase II activity contained in the E. coli strain deposited under accession number DSM 22946; (e) a polynucleotide sequence comprising a cDNA encoding the amino acid sequence consisting of the sequence of amino acids 19-478 of SEQ ID NO:12; (f) a polynucleotide sequence molecule the coding sequence of which differs from the coding sequence of a nucleic acid molecule of any one of (c) to (e) due to the degeneracy of the genetic code; and (g) a polynucleotide sequence encoding a polypeptide having cellobiohydrolase II activity, the polypeptide comprising a heterologous signal sequence linked to an amino acid sequence having at least 80% identity to a sequence consisting of amino acids 19-478 of SEQ ID NO:12.