| 摘要 |
The present disclosure provides methods for the isolation of a cell free complex comprising a circulating, cell free nucleic acid component. The nucleic acid component of the complex is, at least in part, associated with cellular components, such as polypeptides and lipids. The methods of the present disclosure provide for the isolation of circulating cell free nucleic acids that are longer and less fragmented as compared to prior art methods. Furthermore, the circulating, cell free nucleic acid represent biologically relevant nucleic acid targets as the methods described selectively remove non-functional nucleic acids from the isolated material. The nucleic acid component of the isolated complexes reflects a wide spectrum of genomic representation enabling successful detection of mutations, polymorphisms, methylation status and other genomic markers for use in diagnostic and therapeutic applications. Furthermore, the additional cellular components in the cell free complexes isolated provide information regarding the proteomic and lipidomic status of the subject |
