MODIFIED HUMAN ROTAVIRUSES AND USES THEREFOR

摘要

The present disclosure relates generally to the field of viral vaccines. Particularly, the present disclosure provides a modified Vero-adapted human rotavirus strain and a culturing method to produce high titer virus, a rotavirus vaccine, vaccination protocols and diagnostic and prognostic assays.

主权项

1. A method for culturing a strain of rotavirus (RV), said method comprising subjecting an aliquot or inoculum of RV to trypsinization and culturing the trypsinized RV in Vero cells or a Vero cell-derived cell line for a time and under conditions sufficient for the RV to undergo a genetic modification resulting in an amino acid change in one or more of VP1, VP3, VP4, NSP4 and/or NSP5/6 and/or nucleotide change in one or more of VP1, VP3, VP4, NSP2, NSP4 and/or NSP5/6.