| 摘要 |
A method of analysing macromolecular complex ions by mass spectrometry comprises introducing macromolecular complex ions into a first fragmentation device 2, for example a stacked ring ion guide or ion trap (preferably 10-2 to 10-1 mbar), and trapping the complex ions for a trapping period (preferably at least 2 ms). The trapped complex ions are fragmented in the first fragmentation device (preferably at 100 to 300V energy) to produce monomer subunit ions. One or more species of the subunit ions are introduced into a second fragmentation device 6 and fragmented to produce a plurality of fragment ions of the subunit ions. The fragment ions are mass analysed or subjected to further steps of fragmentation before mass analysis. The second fragmentation device may be an ion trap having a higher pressure section for ion fragmentation and a lower pressure section for ion accumulation. The macromolecular complex ions may be protein complexes, DNA-protein complexes, RNA-protein complexes, antibody-drug conjugates or protein-ligand complexes. |
