Method for typing and identification of micro-organisms

摘要

The invention relates to a method for typing or identification of a micro-organism using vibrational spectroscopy, characterised in that, differences in one or more vibrational spectroscopic signal contributions from one or more bleachable components between different spectra obtained of a sample of said micro-organism, and/or between spectra of different samples of said micro-organism, and/or between spectra of samples of different micro-organisms, are substantially eliminated as a source of signal variance in a dataset of said spectra, said bleachable components being molecules possessing vibrational spectral bands that upon exposure to light show a reduction in signal intensity.

主权项

1. A method for typing of a strain of a microorganism belonging to a microbial species, using vibrational spectroscopy, comprising:
Measuring a spectrum of a microorganism strain belonging to said species in a test sample; Prior to said measuring, identifying one or more vibrational spectroscopic signal contributions of bleachable components present in one or more microorganism strains of said species by:
Subjecting each of said one or more strains belonging to said species to a photobleaching process;Recording for each of said one or more strains belonging to said species at least two vibrational spectra at different stages of the photobleaching process; andDetermining for each of said one or more strains belonging to said species the signal contributions of bleachable components by analysis of said spectra obtained at different stages of the photobleaching process; Calculating a corrected spectrum of said microorganism strain in said test sample by substantially eliminating said signal contributions due to bleachable components identified in said determining step from said measured spectrum; and Typing said microorganism strain sample by determining the similarity of said corrected spectrum to a dataset of corrected spectra of microorganism strain samples belonging to said species, wherein signal contributions due to bleachable components have been substantially eliminated from the spectra of said dataset.