Digital microfluidic platform for radiochemistry

摘要

Disclosed herein are methods of performing microchemical reactions and electro-wetting-on-dielectric devices (EWOD devices) for use in performing those reactions. These devices and method are particularly suited for preparing radiochemical compounds, particularly compounds containing 18F.

主权项

1. A method of performing radiosynthesis comprising:
providing an electro-wetting-on-dielectric device (EWOD) comprising:
a first substrate having one or more electrically defined fluid paths along a first surface thereof, each fluid path comprising discrete electrode pads, wherein adjacent electrode pads are separated from each other by non-conductive spaces, each of the electrode pads having electrically conductive lines attached thereto, each conductive line provided to deliver electrical signals to the conductive pad to which the line is attached to provide electrically directed movement of one or more fluid droplets along the fluid paths;one or more fluid delivery sites located on or adjacent to the one or more electrically defined fluid paths; andone or more process operation sites located along the electrically defined fluid paths on the first substrate; placing one or more fluid droplets comprising a radioisotope in a liquid carrier at one of the fluid delivery sites, the delivery site being spaced from the one or more operation sites, providing an electric field in a serial manner to the conductive pads adjacent to the fluid delivery sites to move the one or more fluid droplets comprising the radioisotope to one of the one or more process operation sites; placing one or more fluid droplets comprising at least one radiosynthesis reagent at one of the fluid delivery sites; providing an electric field in a serial manner to the conductive pads adjacent to the fluid delivery sites to move the one or more fluid droplets comprising the radiosynthesis reagent to one of the one or more process operation sites; reacting at least one of the radiosynthesis reagents with the radioisotope at the one or more process operation sites; recovering the reaction product from the EWOD device.