摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to field of molecular biology. Claimed is method for obtaining constant human amniocyte cell line, including transfection of embryonic human amniocyte cells with nucleic acid molecule, which contains nucleic acid sequence, which codes products of adenovirus E1A and E1B genes, with said nucleic acid sequence being obtained from human adenovirus serotype 5, and the following transfection of constant human amniocyte cell line, obtained at first stage, with nucleic acid molecule, containing nucleic acid sequence, coding SV40 large T-antigen.EFFECT: claimed is method for obtaining constant human amniocyte cell line.4 cl, 7 dwg, 9 ex |