Single particle detection device, single particle detection method, and computer program for single particle detection, using optical analysis

摘要

There is provided a single particle detection technique based on the scanning molecule counting method which individually detects single particles using light measurement with a confocal or multiphoton microscope, where the existences of a non-light-emitting particle and a light-emitting particle can be detected while being discriminated from one another in a sample solution. The inventive technique of detecting a single particle detects light from a light detection region during moving the position of the light detection region of the microscope in a sample solution containing a non-light-emitting particle and a light-emitting particle to generate time series light intensity data; and detects in the time series light intensity data a light intensity increase relative to background light intensity as a signal indicating the existence of the light-emitting particle and a light intensity reduction relative to the background light intensity as a signal indicating the existence of the non-light-emitting particle.

主权项

1. A single particle detection device which detects single particles dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising:
a light detection region mover which moves a position of a light detection region of the optical system in the sample solution; a light detector which detects light from the light detection region; and a signal processor which generates time series light intensity data of the light from the light detection region detected with the light detector during moving the position of the light detection region in the sample solution and detects a signal indicating an existence of each single particle individually in the time series light intensity data; wherein the light from the light detection region detected by the light detector includes substantially constant background light; the single particles include a first single particle having an emitting light intensity higher than the background light and a second single particle having an emitting light intensity lower than the background light; the signal of the first single particle is an increase of the light intensity detected by the light detector occurring when the first single particle enters into the light detection region; and the signal of the second single particle is a reduction of the light intensity detected by the light detector occurring when the second single particle enters into the light detection region.