Mutants of a pseudomonas glumae butynol esterase

摘要

The invention relates to novel proteins having esterase activity, to mutants thereof, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

主权项

1. An isolated mutant protein having esterase activity, wherein said isolated mutant protein consists of an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO: 8 and differs from the amino acid sequence of SEQ ID NO: 8 by a mutation in an amino acid position corresponding to any one of residues 12-20 and 185-195 of the amino acid sequence of SEQ ID NO: 8, wherein said isolated mutant protein cleaves the ester but-3-yn-2-yl butyrate, and wherein the isolated mutant protein has a calculated molecular weight of 38 to 34 kDa.