Hybrid portable origin of replication plasmids,申请号US200611990660-传众专利搜索

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发明名称	Hybrid portable origin of replication plasmids
摘要	The invention relates to modifying plasmid origins of replication to create hybrid origins of replication containing nucleotide sequences from more than one plasmid. The invention also relates to a modified origin of replication cassette that is portable or exchangeable due to the creation of multiple cloning sites flanking the origin of replication. Methods and plasmids for use in exchanging origins of replication are disclosed. Such modified or hybrid plasmids provide useful cloning tools that allow for regulation of the level of expression of a desired protein.
申请公布号	US9187752(B2)	申请公布日期	2015.11.17
申请号	US200611990660	申请日期	2006.09.13
申请人	Monsanto Technology LLC	发明人	Bogosian Gregg;O'Neil Julia P.;Smith Hong Q.
分类号	C12N15/09;C12N15/00;C12N15/69	主分类号	C12N15/09
代理机构	Senniger Powers LLP	代理人	Senniger Powers LLP ;Davis James E.
主权项	1. A hybrid origin of replication comprising, at least 200 nucleotides from nucleotide sequences from an origin of replication from a first plasmid contiguously linked to nucleotide sequences from an origin of replication from a second plasmid˜wherein the first plasmid is selected from the group consisting of pBR322, pACYC184, pACYC177, ColE1, pBR3286, pI, pBR26, pBR313, pPIGDM1, pPVUI, pF, pSC101 and pC101p-157, and wherein the origin of replication from the first plasmid and the origin of replication from the second plasmid differ from one another.
地址	St. Louis MO US

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