Nanoparticle mediated delivery of sequence specific nucleases

摘要

Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

主权项

1. A method of introducing a sequence-specific nuclease (SSN) into a plant cell comprising an intact cell wall, the method comprising:
providing the plant cell; coating a nanoparticle with a SSN; placing the plant cell and the coated nanoparticle in contact with each other; and allowing uptake of the nanoparticle and the SSN into the plant cell, wherein the nanoparticle is a gold nanoparticle, a gold coated nanoparticle, or a quantum dot.