RNA APTAMERS AND METHODS FOR IDENTIFYING THE SAME

摘要

RNA aptamers and methods for identifying the same are disclosed. The RNA aptamers selectively bind coagulation factors, E2F family members, Ang1 or Ang2, and therapeutic and other uses for the RNA aptamers are also disclosed.

主权项

1. A method of identifying a ligand which binds to a target, the method comprising:
(a) preparing a candidate oligonucleotide mixture; (b) contacting the candidate oligonucleotide mixture with the target in a low stringency buffer in the presence of a partitioning matrix to produce a binding mixture which comprises a first plurality of ligands which bind the target and a second plurality of ligands which do not bind the target, wherein the first plurality of ligands has increased affinity to the target relative to the second plurality of ligands and/or relative to the partitioning matrix; (c) removing the second plurality of ligands; (d) collecting the ligands that are bound to the target to produce a first collected ligand mixture.