MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS

摘要

A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid encoding a mutant acyl-ACP TE or a chimeric C. viscosissima acyl-ACP TE; a host cell or organism comprising the nucleic acid; a mutant acyl-ACP TE or chimeric C. viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE; and a method of altering the level of activity of a plant acyl-ACP TE.

主权项

1. A method of increasing production of short-chain fatty acids in a host cell or organism, which method comprises introducing into the host cell or organism and expressing therein a nucleic acid molecule comprising a nucleotide sequence encoding an acyl-acyl carrier protein (ACP) thioesterase (TE) from either wild-type Bryantella formatexigens having the amino acid sequence of SEQ ID NO:24 or a mutant acyl-ACP TE derived therefrom, whereupon the production of fatty acids in the host cell or organism is increased.