| 主权项 |
1. A method of cryopreserving a sample, comprising the steps of bringing a sample to be cryopreserved into contact with a cryoprotecting agent to obtain a cryopreservation composition and subsequently reducing the temperature of the cryopreservation composition to a cryopreservation temperature, wherein said cryoprotecting agent comprises one or more cryoprotectants selected from the group consisting of dextrin, dextran, isomaltooligosaccharide, and derivatives thereof which derivative is selected from the group consisting of hydrogenated isomaltooligosaccharide, hydrogenated dextran, hydrogenated dextrin, oxidised isomaltooligosaccharide, oxidised dextran, oxidised dextrin, DEAE-dextrin, DEAE-dextran, DEAE-isomaltooligosaccharides, carboxyC1-10alkyl-dextrin, carboxyC1-10alkyl-dextran, and carboxyC1-10alkyl-isomaltooligosaccharide; and
a) wherein said cryoprotecting agent comprises at least 1% w/w of one or more of isomaltooligosaccharide and derivatives thereof having a weight average molecular weight (Mw) of between 300 and 1,650 Da based on the total weight of dextrin, dextran, isomaltooligosaccharide and derivatives thereof in said cryoprotecting agent, or b) wherein said cryoprotectant has a weight average molecular weight (Mw) of between 300 and 9,500 Da,wherein said sample is selected from the group consisting of mammalian organs, mammalian cells, and mammalian tissues. |
