Compositions and methods for increased ethanol production from biomass

摘要

The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.

主权项

1. A genetically modified yeast cell that overexpresses a xylose transporter polypeptide comprising an amino acid sequence with at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:4.