发明名称 |
Method for isolating osteopontin using feeds containing CMP or casein species |
摘要 |
The present invention pertains to a method for isolating osteopontin from a milk-derived feed containing caseino macropeptide and/or free beta casein, such as e.g. a feed based on milk serum or sweet whey. Particularly, the present method involves the use of a narrow window of pH and specific conductance of the milk-derived feed, which surprisingly has proven to provide a very efficient isolation of osteopontin from chemically complex feeds. |
申请公布号 |
US9181316(B2) |
申请公布日期 |
2015.11.10 |
申请号 |
US201214002578 |
申请日期 |
2012.03.05 |
申请人 |
ARLA FOODS AMBA |
发明人 |
Bertelsen Hans;Wejse Peter Langborg;Trúgvason Trine |
分类号 |
C07K14/52;A23J1/20;C07K1/18;C07K14/47 |
主分类号 |
C07K14/52 |
代理机构 |
Hershkovitz & Associates, PLLC |
代理人 |
Hershkovitz & Associates, PLLC ;Hershkovitz Abe;Aksman Stanislaus |
主权项 |
1. A method for isolating osteopontin from a milk-derived feed, the method comprising the steps of:
a) providing a milk-derived feed comprising osteopontin, said milk-derived feed having a pH in the range of pH 3.6-6.5 at 25 degrees C. and a specific conductance in the range of 4-10 mS/cm at 25 degrees C., and wherein said milk-derived feed furthermore comprises
casein macropeptide in an amount of at least 1% (w/w) relative to the total amount of protein of the milk-derived feed, b) subjecting said milk-derived feed to anion exchange chromatography, which includes contacting the milk-derived feed with an anion exchange medium, c) optionally, washing the anion exchange medium, and d) recovering the protein bound to the anion exchange medium, thereby obtaining a composition comprising isolated osteopontin. |
地址 |
DK |