| 摘要 |
Oxides of lanthanide metals are used to create stable surfaces for use in laser desorption ionization mass spectrometry. The disclosed lanthanide metal oxide surfaces are used to identify and characterize fatty acids from microorganism allowing profiling of the microorganism to the strain level, including antibiotic resistance. Cerium oxide, CeO2, was used to create a stable surface for obtaining fatty acid profiles of bacterial pathogens. Cross validation of results obtained using the disclosed methods, systems, and surfaces, demonstrated greater than 95% accuracy that did not suffer long-term degradation. Bacteria were identified by the disclosed methods, systems, and surfaces with greater than 98% at the species level and 96% at the strain level. Comparisons with existing technologies demonstrate that the presently disclosed methods, systems, and surfaces provide for surprisingly enhanced accuracy and reliability in profiling and identification. |
