Apparatus for enhanced recovery of regenerative cells from tissue samples

摘要

This document describes methods and an apparatus for recovery of a cell-enriched matrix and cells (e.g., regenerative cells) from a tissue sample. In some embodiments, at least two rounds of acceleration and deceleration are performed.

主权项

1. A method for preparing a cell-enriched matrix from a body tissue having vasculature and regenerative cells adaptive to differentiate into functional equivalent of host tissue after transplantation therein, comprising:
extruding a sample of the body tissue through an orifice of a defined diameter in the range of about 1 mm to about 5 mm to reduce lipid content and increase regenerative cell content in the sample, and subjecting the extruded body tissue sample to centrifugation of preselected centrifugal force and duration sufficient to derive therefrom the cell-enriched matrix with regenerative platform therein.