| 摘要 |
<p>An antibiotic-free selection method based on the use of a FabV gene as a selection marker. The FabV gene encodes a triclosan-resistant ACP-enoyl reductase. The FabV gene functionally replaces Fabl in vivo and renders triclosan-sensitive bacteria resistant to triclosan. It can therefore be used as selection marker for plasmid maintenance when triclosan is added as selective agent into the culture medium. This selection method does not require the use of mutant strains or particular media. Moreover triclosan is stable, easy to handle, inexpensive, and approved as safe for humans.</p> |
