ENANTIOSELECTIVE MODIFIED ESTERASE ENZYME AND METHOD FOR THE PRODUCTION THEREOF

摘要

The invention relates to the production of a modified lipolytic enzyme which has been isolated, expressed and purified from heterologous expression. The sequence of the gene encoding for the basal enzyme was obtained from a thermoacidophilic organism of the Acidobacteraceae family. This basal enzyme obtained from a thermoacidophilic organism is able to hydrolyse lipidic substrates (triacylglycerols) bound to medium-chain fatty acids (C6-C10) such as tributyrin, tricaprylin, inter alia. It can also carry out other reactions which are the opposite of hydrolysis, such as synthesis reactions. In addition, this enzyme has enantioselective preference on substrates (2) of esters of profens such as ibuprofen, naproxen and others. The enantioselective lipolytic basal enzyme is modified on the C-terminus thereof in order to add a histidine tail of amino acids which provides it with increased efficiency in the purification process thereof. The invention therefore relates to a method for producing an active pure polypeptide called lipolytic enzyme 499EST obtained by means of the host E. coli BL 21 (DE3).