METHOD FOR PRODUCING A RECOMBINANT PROTEIN OF INTEREST

摘要

Disclosed is a method for producing a recombinant protein of interest, the method being characterised in by the following steps:
(a) providing a fusion protein comprising an N pro autoprotease moiety and a protein of interest moiety in inclusion bodies,
(b) solubilising the inclusion bodies,
(c) allowing the fusion protein to be cleaved by the N pro autoprotease moiety under chaotropic conditions, wherein the recombinant protein of interest is cleaved from the fusion protein and wherein the recombinant protein of interest is not yet renatured or simultaneously renatured, and
(d) recovering the protein of interest, optionally including a renaturing step for the protein of interest.