Recombinant ethanologenic bacteria comprising a urease gene

摘要

The invention provides recombinant ethanologenic bacteria, methods of making the bacteria and methods of producing ethanol using the bacteria.

主权项

1. A recombinant Escherichia coli bacterium comprising a Klebsiella oxytoca urease operon comprising ureD, A, B, C, E, F and G genes, wherein the chromosome of the recombinant Escherichia coli bacterium has a deletion of a methylglyoxal synthase A (mgsA) gene, wherein the nucleotide sequence resulting from the deletion of the mgsA gene on the chromosome of the recombinant Escherichia coli bacterium is SEQ ID NO: 7, and wherein the recombinant Escherichia coli bacterium is ethanologenic and uptakes and metabolizes xylose, arabinose and glucose into ethanol and organic acids during fermentation.