Method for real-time detection of microorganisms in a liquid culture medium using cellular lysis

摘要

The present invention relates to a method for detecting at least one target microorganism that may be present in a sample, comprising the steps of:
a) bringing into contact, in a container: said sample, a medium that enables the growth of the target microorganism(s), and a cell population capable of being lysed by the target microorganism(s);b) subjecting the whole to a temperature that promotes the growth of the target microorganism(s); andc) observing, in real time, lysis of the cell population indicating the presence of the target microorganism(s).;The present invention also relates to a method for detecting and identifying at least one target microorganism that may be present in a sample.

主权项

1. A method for detecting or identifying at least one target microorganism in a sample if present in the sample, comprising:
forming a liquid mixture from at least (i) the sample, (ii) a liquid culture medium that enables the growth of the target microorganism, and (iii) a population of cells or vesicles capable of being lysed by the target microorganism; subjecting the liquid mixture to a temperature that promotes growth of the target microorganism in order to culture the target microorganism if present in the sample; and determining whether the cells or vesicles have been lysed, wherein lysis of the cells or vesicles indicates a positive detection or identification of the target microorganism in the sample.