CO2 PROFILE CULTIVATION

摘要

A dissolved CO2 change in aqueous solutions affects directly the intracellular pH (pHi) value as it does so by influencing therefore important cellular processes. The enzyme carbonic anhydrase II (CAII) catalyzes the equilibrium of CO2 in aqueous solutions and because it alters the speed at which this equilibrium is reached it was identified as a strong candidate for metabolic engineering. The cell line stably expressing hCAII presented a better initial re-alkalinization of cytoplasm after induced CO2 acid load. The most alkaline pHi value associated to the lowest pHi variations was observed for that cell line in long term increased CO2 levels. In general, the increased CO2 profile triggered the quicker progress of G0G1-cell cycle phase for both transfected and control cell lines.

主权项

1. A method for producing a polypeptide comprising the following steps
a) cultivating a mammalian cell comprising a first nucleic acid encoding a carbonic anhydrase and a second nucleic acid encoding the polypeptide, and b) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide.