Modulation of Hematopoietic Stem Cell Differentiation

摘要

The invention provides means of manipulating hematopoietic stem cell differentiation by modulation of levels of NR2F6 (EAR2). Provided are compositions of matter, protocols and methods of use by which inhibiting expression of NR2F6 or activity thereof promotes differentiation of selective hematopoietic lineages, or conversely overexpression of NR2F6 or activity thereof inhibits differentiation. In one embodiment inhibition of differentiation is performed in other cell lineages besides hematopoietic.

主权项

1. A method of accelerating differentiation of a stem cell comprising administration to a subject having a cancerous condition an effective amount of a composition comprising a synthetic oligonucleotide complementary to a nuclear receptor having a mRNA sequence of at least 75% sequence identity to the mRNA sequence of SEQ ID NO: 5 that induces the RNA interference, wherein said nucleotide comprises a sense oligonucleotide strand and an antisense oligonucleotide strand, wherein the sense and antisense oligonucleotide strands form a duplex, and wherein the sense oligonucleotide strand comprises a portion of SEQ ID NO:5 that has been selected based on its ability to inhibits the expression of the nuclear receptor NR2F6 by causing degradation of a ribonucleic acid encoding nuclear receptor NR2F6 by activation of RNA interference.