A multi-analyte detection platform for microdialysis introduces dialysate fluid and one or more reagents into a detection assembly and analyzes the dialysate fluid in near real-time for small or large molecules. The platform uses individually operable actuators to introduce the dialysate fluid and one or more reagents into individual wells of a well plate. An optical assembly detects the color generated from a reaction between the dialysate fluid and one or more reagents. An integrated electrical board performs the functions of the analysis and an onboard monitor provides a user the ability to control the platform through a graphical user interface. The platform is capable of detecting the concentration of different molecules in the dialysate fluid.
