METHODS AND SYSTEMS FOR OPTIMIZING PERFUSION CELL CULTURE SYSTEM

摘要

Methods and perfusion culture systems are disclosed. The systems and methods relate to decreasing the starting perfusion rate, resulting in increased residence time of the cells in the bioreactor and the cell retention device, and/or concomitantly increasing the starting bioreactor volume or decreasing the starting cell retention device volume, or both. Other method embodiments include increasing the concentrations of individual components of the tissue culture fluid, and adding a stabilizer of the degradation of the recombinant protein.

主权项

1. A perfusion bioreactor culture system, comprising:
a bioreactor configured to contain a tissue culture fluid and cells to be cultured; a cell retention device configured to receive tissue culture fluid containing cells from the bioreactor, separate some fluid from the tissue culture fluid and provide harvest output of tissue culture fluid and cells, and provide a recirculation output of tissue culture fluid and cells to the bioreactor; wherein the system has a starting perfusion rate, a starting bioreactor volume, a starting cell retention device volume, and a starting volume ratio of the starting bioreactor volume and the starting cell retention device volume; wherein either the starting perfusion rate is decreased, resulting in increased residence time of the cells in the bioreactor and the cell retention device, or the starting bioreactor volume is increased or the starting cell retention volume is decreased, or both, resulting in an increase in the starting volume ratio.