Bioartificial, primarily vascularized tissue for pharmaceutical tests, method for its production, use of it in a bioreactor.

摘要

Test tissue engineering is bio-artificial tissue with primary blood vessels. The tissue matrix is decellularized, has at least one blood vessel channel and is colonized with human tumor, stem, precursor or liver cells. The tissue is contained in a bioreactor containing culture medium and has an arterial feed and a venous take-off line. Test tissue engineering is bio-artificial tissue with primary blood vessels. The tissue matrix is decellularized, has at least one blood vessel channel and is colonized with human tumor, stem, precursor or liver cells. The bioreactor (1) holds the tissue matrix and/or test tissue (5) within a tissue culture chamber (4) within a vessel (2). The culture medium (15) is delivered through a feed line (6) into the lower section of the vessel. An arterial feed (10) is connected to the arterial blood vessel (13) in the tissue and a venous take-off line (12) is connected to a venous blood vessel (17) in the tissue.